CRISPR Screen Dataset
Findlay S (2018) - 1-PMID30154076
Title: SHLD2/FAM35A co-operates with REV7 to coordinate DNA double-strand break repair pathway choice.
Screen Details:
- Screen Rationale : Response to chemotherapy drug doxorubicin
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Cell Proliferation
- Condition : Doxorubicin (3.0 nM) [ChEBI]
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 129 | Full Dataset Size: 15,606
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.2
- Significance : Authors have indicated that results with Score.1 (Log2FC) < -2.0 OR Score.1 (Log2FC) > 1.97 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors used doxorubicin sensitivity as a proxy to look for genes that may be involved in DNA repair
- A positive logfc score indicates that KO of the gene confers resistance to doxorubicin, a negative logfc score indicates that KO of the gene confers sensitivity to doxorubicin
Score Distribution
< 2.803577983
> -3.995824592