CRISPR Screen Dataset
Lawson KA (2020) - 31-PMID32968282
Title: Functional genomic landscape of cancer-intrinsic evasion of killing by T cells.
Screen Details:
- Screen Rationale : Response to TNF in an ATG12 mutant background
- Cell Type : Mouse Kidney Carcinoma Cell
- Cell Line : RenCa [CELLOSAURUS:CVCL_2174]
- Phenotype : Cell Proliferation
- Condition : Mutation: ATG12 (ETG67526) KO | TNF (10.0 ng/mL)
- Library : mTKO (mouseTKO) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 904 | Full Dataset Size: 19,058
- Experimental Setup : Cytokine exposure | Duration: 15 Days | MOI: ~ 0.5
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a positive Z-score confer increased proliferation in response to TNF upon KO, genes with a negative Z-score confer reduced proliferation in response to TNF upon KO
Score Distribution
< 10.9
> -23.4