CRISPR Screen Dataset
Lawson KA (2020) - 24-PMID32968282
Title: Functional genomic landscape of cancer-intrinsic evasion of killing by T cells.
Screen Details:
- Screen Rationale : Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : B16-MO4 [CELLOSAURUS:CVCL_WM78]
- Phenotype : Cell Proliferation
- Condition : cytotoxic T lymphocytes (CTLs)
- Library : mTKO (mouseTKO) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 116 | Full Dataset Size: 19,060
- Experimental Setup : T cell exposure | Duration: 17 Days | MOI: ~ 0.5
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO.
Score Distribution
< 28.33
> -6.7