CRISPR Screen Dataset
Lawson KA (2020) - 16-PMID32968282
Title: Functional genomic landscape of cancer-intrinsic evasion of killing by T cells.
Screen Details:
- Screen Rationale : Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
- Cell Type : Mammary Gland Tumor Cell Line [BTO:0004086]
- Cell Line : EMT6 [BTO:0003329, CELLOSAURUS:CVCL_1923]
- Phenotype : Cell Proliferation
- Condition : cytotoxic T lymphocytes (CTLs)
- Library : mTKO (mouseTKO) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 624 | Full Dataset Size: 18,986
- Experimental Setup : T cell exposure | Duration: 19 Days | MOI: ~ 0.5
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO.
Score Distribution
< 14.82
> -15.24