CRISPR Screen Dataset
Zou Y (2019) - 3-PMID30962421
Title: A GPX4-dependent cancer cell state underlies the clear-cell morphology and confers sensitivity to ferroptosis.
Screen Details:
- Screen Rationale : Response to ferroptosis inducing chemical
- Cell Type : Renal Cell Carcinoma Cell Line [BTO:0000383]
- Cell Line : 786-O [BTO:0003781, CELLOSAURUS:CVCL_1051]
- Phenotype : Response To Chemicals
- Condition : ML-210 (5 µM) [PubChem]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 1,815 | Full Dataset Size: 23,972
- Experimental Setup : Drug Exposure | Duration: 8 Days
- Significance : Authors have indicated that results with Score.2 (p-Value) > 1.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes with negative log -fold scores resulted in increased sensitivity to the ferroptosis inducing ML-210, KO of hit genes with a positive log-fold score resulted in increased resistance to ferroptosis inducing ML-210
Score Distribution
< 7.254848459
> -4.672054064