CRISPR Screen Dataset
Benslimane Y (2020) - 4-PMID32755594
Title: Genome-Wide Screens Reveal that Resveratrol Induces Replicative Stress in Human Cells.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : T-lymphoblastic Leukemia Cell Line [BTO:0002504]
- Cell Line : Jurkat [BTO:0000661, CELLOSAURUS:CVCL_0065]
- Phenotype : Response To Chemicals
- Condition : Resveratrol (25 µM) [ChEBI]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RANKS | Number of Hits: 1 | Full Dataset Size: 17,821
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.3 (FDR) <= 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes with a negative RANKS score sensitizes cells to resveratrol, KO of hit genes with a positive RANKS score increases resistance to resveratrol
Score Distribution
< 4.74
> -5.3