CRISPR Screen Dataset
Benslimane Y (2020) - 1-PMID32755594
Title: Genome-Wide Screens Reveal that Resveratrol Induces Replicative Stress in Human Cells.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Pre-B Acute Lymphoblastic Leukemia Cell Line [BTO:0000603]
- Cell Line : NALM-6 [BTO:0003288, CELLOSAURUS:CVCL_0092]
- Phenotype : Response To Chemicals
- Condition : Resveratrol (16.0 µM) [ChEBI]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RANKS | Number of Hits: 392 | Full Dataset Size: 19,012
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.2 (FDR) <= 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes with a negative RANKS score sensitizes cells to resveratrol, KO of hit genes with a positive RANKS score increases resistance to resveratrol
Score Distribution
< 3.22
> -5.33