CRISPR Screen Dataset
Zou Y (2020) - 3-PMID32939090
Title: Plasticity of ether lipids promotes ferroptosis susceptibility and evasion.
Screen Details:
- Screen Rationale : Increased resistance to ferroptosis inducing chemical
- Cell Type : Ovarian Cancer Cell Line [BTO:0000811, EFO:0002394]
- Cell Line : OVCAR-8 [BTO:0003054, CELLOSAURUS:CVCL_1629]
- Phenotype : Response To Chemicals
- Condition : 1S,3R-RSL3 (2 µM) [PubChem]
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Kolmogorov-Smirnov | Number of Hits: 232 | Full Dataset Size: 18,663
- Experimental Setup : Drug Exposure | Duration: 12 Days | MOI: < 1
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of the hit gene increases resistance to ferroptosis inducing RSL3
Score Distribution
< 0.049401649
> 0.0000034