CRISPR Screen Dataset
Wang R (2020) - 3-PMID33333024
Title: Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : Huh-7.5.1 [BTO:0005463, CELLOSAURUS:CVCL_E049]
- Phenotype : Response To Virus
- Condition : Virus: HCoV-229E (Human coronavirus 229E) (0.05 MOI) [NCBI Taxid 11137]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 597 | Full Dataset Size: 20,915
- Experimental Setup : Virus Exposure | Duration: 10 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 0.005 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased resistance to HCoV-229E
Score Distribution
< 1
> 7.91e-27