CRISPR Screen Dataset
Wang R (2020) - 1-PMID33333024
Title: Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : Huh-7.5.1 [BTO:0005463, CELLOSAURUS:CVCL_E049]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) (0.01 MOI) [NCBI Taxid 2697049]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 431 | Full Dataset Size: 20,915
- Experimental Setup : Virus Exposure | Duration: 12 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 0.005 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased resistance to SARS-CoV-2
- Huh7.5.1 cells harboring a bicistronic ACE2-IRES-TMPRSS2 construct were used as these cells sustained efficient infection that led to widespread cell death, while still allowing the survival of a small number of cells
Score Distribution
< 1
> 0.00000000000457