CRISPR Screen Dataset
Dukhovny A (2019) - 1-PMID31142663
Title: A CRISPR Activation Screen Identifies Genes That Protect against Zika Virus Infection.
Screen Details:
- Screen Rationale : Activation increases resistance to the virus
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
- Phenotype : Response To Virus
- Condition : Virus: Zika virus (0.2 MOI) [NCBI Taxid 64320]
- Library : Human SAM library in lentiSAMv2 backbone [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 936 | Full Dataset Size: 18,434
- Experimental Setup : Virus Exposure | Duration: 10 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- activation of the hit gene increases cell proliferation in the presence of Zika virus
Score Distribution
< 0.99996
> 0.000000000737