CRISPR Screen Dataset
Arroyo JD (2016) - 1-PMID27667664
Title: A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
Screen Details:
- Screen Rationale : Cell-essential genes for growth in galactose
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Viability [APO:0000111]
- Condition : Galactose medium
- Library : Avana-4 | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 191 | Full Dataset Size: 19,632
- Experimental Setup : Timecourse | Duration: 12 Days
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Authors used Annexin V to select for dead cells and sequence them for enriched sgRNAs, thus using positive selection to identify cell-essential genes in galactose medium
Score Distribution
< 19632
> 1