CRISPR Screen Dataset
Liu N (2018) - 2-PMID29211708
Title: Selective silencing of euchromatic L1s revealed by genome-wide screens for L1 regulators.
Screen Details:
- Screen Rationale : Regulators of L1 retrotransposition
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 15 | Full Dataset Size: 20,514
- Experimental Setup : Timecourse | Duration: 18 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.3 (q-Value) <= 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- L1-G418R retrotransposition reporter
- The cells become G418R antibiotic-resistant only when the L1-G418R reporter undergoes a successful retrotransposition event after dox induction
- cells transduced with sgRNAs targeting L1 suppressors would have more retrotransposition events than negative control cells and would be enriched through the G418 selection; conversely, cells transduced with sgRNAs targeting L1 activators would be depleted.
Score Distribution
< 53.4
> 0