CRISPR Screen Dataset
Jost M (2017) - 1-PMID28985505
Title: Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent.
Screen Details:
- Screen Rationale : Gene inhibition affects response to chemical
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Response To Chemicals
- Condition : rigosertib (900.0 nM) [ChEBI]
- Library : CRISPRi (Weissman, 2014) [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 619 | Full Dataset Size: 31,977
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: < 1.0
- Significance : Authors have indicated that results with Score.1 (Rho (Log2e Treated vs. Untreated)) > 0.15 OR Score.1 (Rho (Log2e Treated vs. Untreated)) < -0.15 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- inhibition of hit genes results in decreased sensitivity to rigosertib (positive rho score) or increased sensitivity to rigosertib (negative rho score)
Score Distribution
< 0.803637619
> -0.411213056