CRISPR Screen Dataset
Savidis G (2016) - 1-PMID27342126
Title: Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Cell Line : H1HeLa [CLO:0003586, CELLOSAURUS:CVCL_3334]
- Phenotype : Response To Virus
- Condition : Virus: Zika virus (5 MOI) [NCBI Taxid 64320]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : None | Number of Hits: 756 | Full Dataset Size: 19,050
- Experimental Setup : Virus Exposure | Duration: 8 Days | MOI: = 0.2
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of hit genes increases survival of H1-HeLa cells exposed to Zika virus
Score Distribution
< 6
> 3