CRISPR Screen Dataset
Hyrina A (2019) - 4-PMID31801065
Title: A Genome-wide CRISPR Screen Identifies ZCCHC14 as a Host Factor Required for Hepatitis B Surface Antigen Production.
Screen Details:
- Screen Rationale : Increased hepatitis B virus antigen surface expression ( HBsAg) of an HBV genome integrated cell in the presence of RG7834, a small molecule inhibitor of HBV replication
- Cell Type : Hepatitis B Virus Genome Integrated Cell Line
- Cell Line : HepG2-NTCP1 HBV Cas9 [CELLOSAURUS:CVCL_C9BB]
- Phenotype : Response To Virus
- Condition : RG7834 (30 nM) [PubChem]
- Library : CRISPRn (Helliwell, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 3 | Full Dataset Size: 19,049
- Experimental Setup : Drug Exposure | Duration: 10 Days | MOI: = 0.05
- Significance : Authors have indicated that results with Score.2 (RSA) < -5.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes suppresses the activity of RG7834, a small molecule inhibitor of HBV replication
Score Distribution
< 0.837403102
> -0.516763478