CRISPR Screen Dataset
Hyrina A (2019) - 1-PMID31801065
Title: A Genome-wide CRISPR Screen Identifies ZCCHC14 as a Host Factor Required for Hepatitis B Surface Antigen Production.
Screen Details:
- Screen Rationale : Pro-HBsAg (hepatitis B virus surface antigen) expression genes
- Cell Type : Hepatitis B Virus Genome Integrated Cell Line
- Cell Line : HepG2-NTCP1 HBV Cas9 [CELLOSAURUS:CVCL_C9BB]
- Phenotype : Response To Virus
- Library : CRISPRn (Helliwell, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 22 | Full Dataset Size: 19,049
- Experimental Setup : Timecourse | Duration: 10 Days | MOI: = 0.05
- Significance : Authors have indicated that results with Score.1 (Log2) < -1.018 AND Score.2 (RSA) < -4.94 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- authors stained Hep-G2(HBV) cells with an HBsAg antibody and sorted into high HBsAg and low HBsAg populations
- enrichment of sgRNA-targeted genes was compared between populations expressing high and low levels of HBsAg. This analysis identified 22 genes that appeared to promote HBsAg expression ("pro-HBsAg")
Score Distribution
< 1.124784142
> -1.733154678