CRISPR Screen Dataset
Gilbert LA (2014) - 4-PMID25307932
Title: Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Screen Details:
- Screen Rationale : Gene activation affects response to toxin
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Response To Toxin
- Condition : CTx-DTA (0.4 nM)
- Library : CRISPRa (Weissman, 2014) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: sunCas9 | Methodology: Activation
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 132 | Full Dataset Size: 15,977
- Experimental Setup : Toxin Exposure | Duration: 10 Days | MOI: < 1.0
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- negative rho scores indicate activation of the gene has a sensitizing effect on cells exposed to CTx-DTA, positive who scores indicate activation of the gene has a protective effect on cells exposed to CTx-DTA
Score Distribution
< 0.711621998
> -0.751386767