CRISPR Screen Dataset
Gilbert LA (2014) - 1-PMID25307932
Title: Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Screen Details:
- Screen Rationale : Gene inhibition affects response to toxin
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Response To Toxin
- Condition : CTx-DTA (0.4 nM)
- Library : CRISPRi (Weissman, 2014) [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 457 | Full Dataset Size: 15,976
- Experimental Setup : Toxin Exposure | Duration: 10 Days | MOI: < 1.0
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- positive rho score shows that inhibition of the gene has a protective effect on cells exposed to CTx-DTA, negative rho score indicates that inhibition of the gene has a sensitizing effect on cells exposed to CTx-DTA toxin
Score Distribution
< 1.079970899
> -0.864063861