CRISPR Screen Dataset
Pech MF (2019) - 2-PMID31452512
Title: Systematic identification of cancer cell vulnerabilities to natural killer cell-mediated immune surveillance.
Screen Details:
- Screen Rationale : Cancer cell response to natural killer cells
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Condition : NK (activated natural killer) cells (250.0 M)
- Library : CRISPRn (Settleman, 2019) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 22 | Full Dataset Size: 20,454
- Experimental Setup : NK cell exposure | Duration: 8 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (FDR) <= 0.1 OR Score.4 (FDR) <= 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- authors screened for genes whose loss sensitized/protected K-562 cells to natural killer cells
Score Distribution
< 1
> 0.000000000363