CRISPR Screen Dataset
Pan D (2018) - 1-PMID29301958
Title: A major chromatin regulator determines resistance of tumor cells to T cell-mediated killing.
Screen Details:
- Screen Rationale : Tumor response to cytotoxic T-cells
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : B16-F10 [BTO:0002014, CELLOSAURUS:CVCL_0159]
- Phenotype : Cell Proliferation
- Condition : Pmel-1 T cells
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 325 | Full Dataset Size: 19,674
- Experimental Setup : T cell exposure | Duration: 3 Days | MOI: = 0.06
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- The authors screened for gene knockouts in a melanoma cell line that affect sensitivity to killing by cytotoxic Pmel-1 T cells, which have a relatively low TCR affinity for an endogenous melanoma antigen
- Knock out of genes with a positive log fold change score results in increased resistance to Pmel-1 T cells. A negative log fold change score indicates that knock out of the gene resulted in increased sensitivity to Pmel-1 cells
Score Distribution
< 9.69
> -3.93