CRISPR Screen Dataset
Deans RM (2016) - 1-PMID27018887
Title: Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Response To Chemicals
- Condition : GSK983 (48.0 nM) [PubChem]
- Library : CRISPRn (Bassik, 2016) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 1,467 | Full Dataset Size: 18,506
- Experimental Setup : Drug Exposure | Duration: 14 Days
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by ' ' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- Genes with a negative log2 score show increased sensitivity to the anti-viral drug GSK983 upon KO, genes with a positive log2 score show increased resistance to the anti-viral drug GSK983 upon KO
Score Distribution
< 2.609257642
> -2.546629654