CRISPR Screen Dataset
Han J (2018) - 1-PMID29642015
Title: Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A-549 [BTO:0000018, CELLOSAURUS:CVCL_0023]
- Phenotype : Response To Virus
- Condition : Virus: Influenza A virus (A/Viet Nam/1203/2004(H5N1)) (5 MOI) [NCBI Taxid 284218]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 798 | Full Dataset Size: 19,050
- Experimental Setup : Virus Exposure | Duration: 4 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- cells were exposed to 2 rounds of infection with recovery time between rounds
Score Distribution
< 0.094171
> 0.00000000443