CRISPR Screen Dataset
Dhoonmoon A (2020) - 1-PMID32542389
Title: Genome-wide CRISPR synthetic lethality screen identifies a role for the ADP-ribosyltransferase PARP14 in DNA replication dynamics controlled by ATR
Screen Details:
- Screen Rationale : Cell-essential genes (PARP14-deficient cells)
- Cell Type : Pancreatic Adenocarcinoma Cell Line [BTO:0001521]
- Cell Line : PaTu 8988t [CLO:0008384, CELLOSAURUS:CVCL_1847]
- Phenotype : Cell Proliferation
- Condition : PARP14 knockout
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 600 | Full Dataset Size: 19,112
- Experimental Setup : Timecourse | Duration: 2 Weeks | MOI: = 0.4
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- The authors noted that multiple components of the ATR DNA replication stress pathway were present in the top 600 genes ranked by the RSA analysis. They further investigated four genes among the top 600, in particular CHK1, TOPBP1, MRE11, and DNA2. This is a synthetic lethality screen with PARP14-knockout cells.
Score Distribution
< 1
> -6.976905492