CRISPR Screen Dataset
Jain IH (2020) - 2-PMID32259488
Title: Genetic Screen for Cell Fitness in High or Low Oxygen Highlights Mitochondrial and Lipid Metabolism.
Screen Details:
- Screen Rationale : Increased sensitivity to low oxygen
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Condition : Low oxygen (1.0 %)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 109 | Full Dataset Size: 20,112
- Experimental Setup : Oxygen Exposure | Duration: 15 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (p-Value) <= 6.28e-05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- gene knockouts show a decreased fitness in low oxygen conditions (1%) compared to high oxygen conditions (21%) after 15 days
Score Distribution
< 1
> 0.000000246