CRISPR Screen Dataset
Breslow DK (2018) - 1-PMID29459677
Title: A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Screen Details:
- Screen Rationale : Regulators of Hh signaling pathway
- Cell Type : Embryonic Fibroblast Cell Line [BTO:0001958]
- Cell Line : NIH 3T3 [BTO:0000944, CELLOSAURUS:CVCL_0594]
- Phenotype : Regulation Of Signal Transduction Phenotype [CMPO:0000040]
- Condition : Sonic hedgehog protein (mouse) [Uniprot]
- Library : Bassik Mouse CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 472 | Full Dataset Size: 20,898
- Experimental Setup : Ligand Exposure | Duration: 5 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.4 (FDR) = 1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors screened for genes affecting ciliary Hh signaling using a a reporter that converts Hh signaling into antibiotic resistance. Genes with a positive CasTLE effect increase Hh pathway signaling while genes with a negative CasTLE effect decrease Hh pathway signaling
Score Distribution
< 237
> 0