CRISPR Screen Dataset
Leto DE (2019) - 4-PMID30581143
Title: Genome-wide CRISPR Analysis Identifies Substrate-Specific Conjugation Modules in ER-Associated Degradation.
Screen Details:
- Screen Rationale : ERAD-L genes
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 565 | Full Dataset Size: 20,502
- Experimental Setup : Timecourse | Duration: 20 Hours
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- the authors used a GFP-RTA-E177Q tagged reporter to screen for genes whose loss caused an increase in GFP levels, indicating ERAD was disrupted
Score Distribution
< 543
> 0