CRISPR Screen Dataset
Shifrut E (2018) - 2-PMID30449619
Title: Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function.
Screen Details:
- Screen Rationale : T cell proliferation genes or proliferation inhibition genes
- Cell Type : Regulatory T Cell [CL:0000815]
- Cell Line : CD8+ T s (primary-derived)
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 3,787 | Full Dataset Size: 19,114
- Experimental Setup : Timecourse | Duration: 6 Days
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- in order to screen primary human T cells the authors introduce traceable sgRNA cassettes by lentivirus followed by electroporation with Cas9 protein, this technique is termed SLICE in their paper
- authors use fluorescent dye (CFSE) to differentiate proliferating cells
Score Distribution
< 1
> 0.0000000000215