CRISPR Screen Dataset
Panganiban RA (2019) - 1-PMID31213543
Title: Genome-wide CRISPR screen identifies suppressors of endoplasmic reticulum stress-induced apoptosis.
Screen Details:
- Screen Rationale : ER stress response genes
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Sodium Arsenite (5.0 µM) [ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 361 | Full Dataset Size: 19,050
- Experimental Setup : Drug Exposure | Duration: 30 Hours | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- authors used a fluorescence reporter (mCherry) under the control of the CHOP gene promoter to identify cells with altered response to ER stressor As
- suppressors of the ER stress response show elevated flourescence in a KO screen
Score Distribution
< 7.4
> 2.5