CRISPR Screen Dataset

Doench JG (2016) - 13-PMID26780180

H. sapiens

Title: Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.

Screen Rationale : Increased drug resistance
Cell Type : Melanoma Cell Line [BTO:0000849]
Cell Line : A-375 [BTO:0002806, CELLOSAURUS:CVCL_0132]
Phenotype : Response To Chemicals
Condition : Selumetinib (200.0 nM) [ChEBI]
Library : Avana | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : STARS | Number of Hits: 97 | Full Dataset Size: 18,675
Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: < 1.0
Significance : Authors have indicated that results with Score.2 (FDR) < 0.1 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Phenotypic readout: cell proliferation
With the Avana library screened in lentiCRISPRv2, 97 genes showed selumetinib resistance (A-375 cells; STARS analysis at FDR < 10%).

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