CRISPR Screen Dataset

Jeng EE (2019) - 1-PMID31540829

H. sapiens

Title: Systematic Identification of Host Cell Regulators of Legionella pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.

Screen Rationale : Increased resistance or sensitivity to bacteria
Cell Type : Lymphoma Cell Line [BTO:0000104]
Cell Line : U-937 [BTO:0001412, CELLOSAURUS:CVCL_0007]
Phenotype : Response To Bacteria
Condition : Legionella pneumophila (100.0 MOI) [NCBI Taxid 446]
Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : CasTLE | Number of Hits: 186 | Full Dataset Size: 20,561
Experimental Setup : Bacteria Exposure | Duration: 18 Days
Significance : Authors have indicated that results with Score.4 (FDR) = 1.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Phenotypic readout: cell proliferation
A negative CASTLE effect indicates increased sensitivity to L. pneumophila, a positive CASTLE effect indicates increased resistance to L. pneumophila
Hit genes are those that met a minimum FDR value of 10%

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