CRISPR Screen Dataset
Zhuang X (2019) - 2-PMID31921143
Title: Genome-Wide CRISPR Screen Reveals Cancer Cell Resistance to NK Cells Induced by NK-Derived IFN-γ.
Screen Details:
- Screen Rationale : Cancer cell response to natural killer cells
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Condition : IL-2-activated NK cells (0.3 Ratio of effector cells/target cells)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 5,829 | Full Dataset Size: 20,697
- Experimental Setup : NK cell exposure | Duration: 2 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- enes with a negative beta score indicate knock-out of the gene results in increased sensitivity to natural killer cells, genes with a positive beta score indicate knock-out of the gene shows reduced sensitivity to natural killer cells
Score Distribution
< 1.2477
> -1.4187