CRISPR Screen Dataset
Yamaji T (2019) - 1-PMID30660999
Title: A CRISPR Screen Identifies LAPTM4A and TM9SF Proteins as Glycolipid-Regulating Factors.
Screen Details:
- Screen Rationale : Regulation of Gb3 biosynthesis
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Response To Toxin
- Condition : Shiga Toxin Stx1 (50 M)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 97 | Full Dataset Size: 19,050
- Experimental Setup : Toxin Exposure | Duration: 14 Days | MOI: ~ 0.2
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Phenotypic readout: cell proliferation
- the authors used Shiga toxin resistance to screen for regulators of biosynthesis of the Shiga toxin receptor, globotriaosylceramide (Gb3). Genes whose loss inhibit the activity of Gb3 prevent Shiga toxin induced death and are enriched.
Score Distribution
< 1092
> 1