CRISPR Screen Dataset
Villegas F (2019) - 1-PMID30595499
Title: Lysosomal Signaling Licenses Embryonic Stem Cell Differentiation via Inactivation of Tfe3.
Screen Details:
- Screen Rationale : Regulators of differentiation
- Cell Type : Mouse Embryonic Stem Cell [EFO:0004038]
- Cell Line : RGd2 ES
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Differentiation conditions (absence of 2i)
- Library : Murine lentiviral gRNA library (version 1) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 18 | Full Dataset Size: 19,138
- Experimental Setup : Timecourse | Duration: 3 Days | MOI: = 0.25
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.25 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- authors used the presence of GFP-tagged Rex1 (a self-renewing ESC reporter) to screen for genes whose loss prevented differentiation
Score Distribution
< 1
> 3.11e-16