CRISPR Screen Dataset

Patil A (2019) - 2-PMID31300418

H. sapiens

Title: Genome-wide CRISPR screens reveal genetic mediators of cereblon modulator toxicity in primary effusion lymphoma.

Screen Rationale : Increased drug resistance
Cell Type : Primary Effusion Lymphoma Cell Line [BTO:0002719]
Cell Line : BC-3 [BTO:0002722, CELLOSAURUS:CVCL_1080]
Phenotype : Response To Chemicals
Condition : CC-122 (Avadomide) (1.0 µM) [ChEMBL, ChemSpider]
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 1 | Full Dataset Size: 19,049
Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: ~ 0.3
Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Phenotypic readout: cell proliferation
The authors performed positive selection screens to identify Cereblon modulating agent (CM) effectors, genes whose inactivation confers resistance to LEN, POM, or CC-122 in PEL cells.

< 0.99998

> 0.000000000000118