CRISPR Screen Dataset
Xiao T (2018) - 2-PMID29987050
Title: Estrogen-regulated feedback loop limits the efficacy of estrogen receptor-targeted breast cancer therapy.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Breast Cancer Cell Line [BTO:0000356, EFO:0002885]
- Cell Line : T-47D [BTO:0001248, CELLOSAURUS:CVCL_0553]
- Phenotype : Cell Proliferation
- Condition : E2 (17β-estradiol) (10.0 nM) [ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,036 | Full Dataset Size: 18,567
- Experimental Setup : Ligand Exposure | Duration: 4 Weeks
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors screened breast cancer cell lines in the presence and absence of estrogen to look for genes whose loss could confer growth in the absence of estrogen and genes whose loss affects cell viability.
Score Distribution
< 0.96516
> -1.6211