CRISPR Screen Dataset

Kabir S (2019) - 2-PMID31294695

H. sapiens

Title: The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells.

Screen Rationale : Gene inhibition increases drug sensitivity
Cell Type : Lung Squamous Cell Carcinoma Cell Line [BTO:0002202, EFO:0000708]
Cell Line : LK-2 [EFO:0003141, CELLOSAURUS:CVCL_1377]
Phenotype : Response To Chemicals
Condition : AZD5991 (MCL1i) (1.0 µM)
Library : CRISPRi-v2 (inhibition) [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
Analysis Method : MaGeCK | Number of Hits: 17 | Full Dataset Size: 18,788
Experimental Setup : Drug Exposure | Duration: 12 Hours | MOI: ~ 0.3
Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 AND Score.3 (FDR) < 0.25 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Phenotypic readout: cell proliferation
Inhibition of hit genes resensitizes LK-2 cells to MCL1 inhibition as determined by loss of viability measured by level of apoptosis (FACS sorting based on presence of fluorescent marker)

< 1

> 0.0000000077