CRISPR Screen Dataset
Jiang C (2019) - 2-PMID31365872
Title: CRISPR/Cas9 Screens Reveal Multiple Layers of B cell CD40 Regulation.
Screen Details:
- Screen Rationale : Positive regulators of CD40 pathway
- Cell Type : Burkitt Lymphoma Cell Line [BTO:0000889]
- Cell Line : Daudi [BTO:0001561, CELLOSAURUS:CVCL_0008, DEPMAP:ACH-000786]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Mega CD40L (50 ng/mL) [Uniprot, Enzo]
- Library : Avana | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 85 | Full Dataset Size: 18,675
- Experimental Setup : Ligand Exposure | Duration: 48 Hours | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Daudi B cells were transfected with the Avana sgRNA library and then stimulated with trimeric Mega-CD40L. CD40-induced Fas levels were analyzed by fluorescence-activated cell sorting (FACS), cells with the lowest FAS abundance were determined to likely contain knockouts of CD40 pathway positive regulators.
Score Distribution
< 14.90846792
> 4.182324388