CRISPR Screen Dataset

Adhikari H (2018) - 1-PMID30194290

H. sapiens

Title: Interrogating the protein interactomes of RAS isoforms identifies PIP5K1A as a KRAS-specific vulnerability.

Screen Rationale : Cell-essential genes (HRASG12V isoform)
Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
Cell Line : HEK-HT [CELLOSAURUS:CVCL_C9AL]
Phenotype : Cell Proliferation
Condition : HRASG12V isoform
Library : RAS Interactome (Adhikari, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : EdgeR | Number of Hits: 166 | Full Dataset Size: 476
Experimental Setup : Timecourse | Duration: 21 Days | MOI: ~ 0.3
Significance : Authors have indicated that results with Score.1 (Log2) < -0.5 AND Score.2 (p-Value) < 0.05 AND Score.3 (FDR) < 0.01 OR Score.1 (Log2) > 0.5 AND Score.2 (p-Value) < 0.05 AND Score.3 (FDR) < 0.01 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A focused RAS Interactome library targeting 476 genes was generated. Any gene with a log2 fold change < -0.5 and a p-value < 0.05 with an FDR < 0.01 was considered to be a negatively enriched or depleted gene hit, whereas any gene with a log2 fold change > 0.5 and a p-value < 0.05 with an FDR < 0.01 was considered to be a positively enriched gene hit. The HEK-HT cells are expressing an oncogenic version of one RAS isoform. This HRAS screen involved HEK-HT cells with the HRASG12V isoform.

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