Featured CRISPR Screens: Three New CRISPR-Cas9 Screens Exploring Human Host Factors for SARS-CoV-2 and Common Cold Coronaviruses from Wang R et. al (2020)

Our latest BioGRID ORCS CRISPR Database update adds 3 new screens from the preprint study, "Functional genomic screens identify human host factors for SARS-CoV-2 and common cold coronaviruses" by Wang R et al. (BioRxiv, 2020.09.24.312298). In this preprint, the authors performed CRISPR screens in cells infected by SARS-CoV-2 as well as two seasonally circulating common cold coronaviruses, OC43 and 229E. These screens identified nearly 200 host proteins and their related pathways which may support coronavirus viral infection in a manner shared by mulitple members of the Coronaviridae family and over 390 genes whose loss reduced SARS-CoV-2 infection specifically. General host factors included SCAP activity and suggest cholesterol levels may play a role in coronavirus progression, which is consistent with recent interaction data published in the literature. The results of this study also suggest that the poorly characterized TMEM106B gene may play a role in SARS-CoV-2 progression exclusively.

A special thanks to the authors Wang R, Simoneau CR, Kulsuptrakul J, Bouhaddou M, Travisano K, Hayashi JM, Carlson-Stevermer J, Oki J, Holden K, Krogan NJ, Ott M, and Puschnik AS and the Chan Zuckerberg Biohub, Synthego, Gladstone Institutes, and UCSF Quantitative Biosciences Institute.

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October 20th, 2020 - 10:13 PM