Featured Dataset: A new Human CRISPR-Cas9 Screen Exploring Essential Genes in PARP14-deficient Cells from Dhoonmoon A et. al (2020)

Our latest BioGRID ORCS CRISPR Database update adds a new screen from the study, "Genome-wide CRISPR synthetic lethality screen identifies a role for the ADP-ribosyltransferase PARP14 in DNA replication dynamics controlled by ATR" by Dhoonmoon A et al. (Nucleic Acids Res, 2020). For this dataset, a genome-wide CRISPR knockout screen was carried out in PARP14-deficient cells identifying synthetic lethal interactors of PARP14, including several components of the ATR DNA replication stress pathway. The ATR-CHK1 pathway was found to be essential for viability of PARP14-deficient cells. This indicates that the status of the PARP14 gene in a tumor may be an important determinant of its response to DNA damage response (DDR)inhibitors, such as ATR–CHK1 pathway inhibitors which are being developed as anti-cancer drugs.

A special thanks to the authors Dhoonmoon A, Schleicher EM, Clements KE, Nicolae CM and Moldovan GL and the Department of Biochemistry & Molecular Biology at PennState. We'd also like to specifically thank author George-Lucian Moldovan for working with us to ensure this dataset was deposited directly into our CRISPR Database.

If you have a CRISPR Screen Dataset you'd like to deposit directly into the BioGRID ORCS CRISPR Database, please contact us at

August 18th, 2020 - 02:19 PM