CRISPR Screen Dataset
Behan FM (2019) - 80-PMID30971826
Title: Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Lung Squamous Cell Carcinoma Cell Line [BTO:0002202, EFO:0000708]
- Cell Line : HARA [CLO:0009998, CELLOSAURUS:CVCL_2914]
- Phenotype : Cell Proliferation
- Library : Human CRISPR Library v.1.1 | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 1,708 | Full Dataset Size: 17,995
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 0.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a Scaled Bayesian Factor (BF) greater than 0 were identified as fitness genes (hits) for this cell line in this CRISPR screen. The modified BAGEL analysis computed gene-level BFs by the sgRNAs by averaging them instead of summing them. A 5% FDR threshold was used.
Score Distribution
< 4.73936650942227
> -2.56284910390635