CRISPR Screen Dataset
Yamauchi T (2018) - 1-PMID29478914
Title: Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : MLL/AF9 Cas9 [CELLOSAURUS:CVCL_C8ZF]
- Phenotype : Cell Proliferation
- Library : GECKO v2 (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,714 | Full Dataset Size: 20,611
- Experimental Setup : Timecourse | Duration: 16 Days
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- authors reported genes that met a cut-off of FDR<0.25 as determined my Mageck
Score Distribution
< 0.249814
> 0.00165