CRISPR Screen Dataset

Liao S (2018) - 1-PMID30135075

H. sapiens

Title: Genetic modifiers of the BRD4-NUT dependency of NUT midline carcinoma uncovers a synergism between BETis and CDK4/6is.

Screen Rationale : Increased drug resistance
Cell Type : NMC-G1 Cell [CLO:0009897]
Cell Line : 10-15 [CELLOSAURUS:CVCL_D1H0]
Phenotype : Response To Chemicals
Condition : JQ1 (200 nM) [ChEBI]
Library : CRISPRn Targeted Library (Elledge, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 11 | Full Dataset Size: 497
Experimental Setup : Drug Exposure | Duration: 17 Days | MOI: = 0.2
Significance : Authors have indicated that results with Score.4 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Phenotypic readout: cell proliferation
Authors use a targeted tumor suppressor gene (TSG) library in a NUT midline carcinoma cell line to determine which alterations could substitute for BRD4-NUT signaling using a chemical inhibitor of BRD4: JQ1

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