CRISPR Screen Dataset
Manguso RT (2017) - 3-PMID28723893
Title: In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target.
Screen Details:
- Screen Rationale : Tumorigenicity in presence of immunotherapy
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : B16-F10 [BTO:0002014, CELLOSAURUS:CVCL_0159]
- Phenotype : Tumorigenicity
- Condition : Immunotherapy (GVAX only)
- Library : CRISPRn Targeted Library (Haining, 2017) | Type: CRISPRn | Format: in vivo | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 55 | Full Dataset Size: 2,368
- Experimental Setup : Implantation to Mouse Model | Duration: 12 Days
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- sgRNAs that are significantly depleted from tumours treated with the immunotherapy cancer vaccine GVAX relative to TCRalpha KO mice which lack CD4+ and CD8+ T cells and are unable to apply adaptive immune-selective pressure on the tumours, indicate that loss of the targeted gene increased the sensitivity of tumour cells to GVAX
- GVAX is a vaccine composed of whole tumor cells genetically modified to secrete the immune stimulatory cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), and then irradiated to prevent further cell division to generate an adaptive immune response sufficient to apply immune-selective pressure on tumour cells
Score Distribution
< 8.572587192
> 0.813740781