CRISPR Screen Dataset
Uijttewaal ECH (2024) - 2-PMID39681701
Title: CRISPR-StAR enables high-resolution genetic screening in complex in vivo models.
Screen Details:
- Screen Rationale : Cell-essential and growth restrictive genes in established tumors in vivo
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : YUMM1.7 [CELLOSAURUS:CVCL_JK16]
- Phenotype : Cell Proliferation
- Library : CRISPRn library in CRISPR-StAR vector (Elling, 2024) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CRISPR-StAR–MAGeCK analysis pipeline | Number of Hits: 515 | Full Dataset Size: 20,461
- Experimental Setup : Implantation to Mouse Model | Duration: 14 Days | MOI: = 0.25
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- The authors screened for genes whose KO resulted in decreased (negative log2fc) or increased (positive log2fc) proliferation in established tumors from Yumm1.7 450R mouse melanoma cells injected into Rag2-/- mouse models using CRISPR-StAR.
- To select for genes whose dependency is specific for in vivo conditions genes with an in vitro log2fc <-1.5 have been filtered out from the list of hits
Score Distribution
< 5.0076
> -8.1722