CRISPR Screen Dataset
Uijttewaal ECH (2024) - 1-PMID39681701
Title: CRISPR-StAR enables high-resolution genetic screening in complex in vivo models.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : YUMM1.7 [CELLOSAURUS:CVCL_JK16]
- Phenotype : Cell Proliferation
- Library : CRISPRn library in CRISPR-StAR vector (Elling, 2024) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CRISPR-StAR–MAGeCK analysis pipeline | Number of Hits: 477 | Full Dataset Size: 20,461
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: = 0.25
- Significance : Authors have indicated that results with Score.1 (Log2FC) < -3.0 AND Score.2 (RRA score) >= 10.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors screened for genes whose KO resulted in decreased proliferation in the Yumm1.7 450R mouse melanoma cell line using CRISPR-StAR
Score Distribution
< 1.8429
> -7.0383