CRISPR Screen Dataset
Ruan Y (2023) - 1-PMID37059858
Title: A multi-omics integrative analysis based on CRISPR screens re-defines the pluripotency regulatory network in ESCs.
Screen Details:
- Screen Rationale : Regulation of embryonic stem cell renewal
- Cell Type : Embryonic Stem Cell Line [BTO:0001581]
- Cell Line : R1 [BTO:0004500]
- Phenotype : Cell Proliferation
- Condition : Leukaemia inhibitory factors (LIF)/serum condition (L/S)
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 4,342 | Full Dataset Size: 19,674
- Experimental Setup : Other | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes whose sgRNAs are significantly depleted (negative MAGeCK score) are required for mESC fitness
- Genes whose sgRNAs were enriched (positive MAGeCK score) inhibit the self-renewal of mESCs
Score Distribution
< 1
> 0.000000000369