CRISPR Screen Dataset
Levi O (2025) - 1-PMID40852982
Title: Genome-wide CRISPRi screen and proteomic profiling identify key genes related to ferulic acid's antifungal activity.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Saccharomyces Cerevisiae [NCBI Taxid 4932]
- Cell Line : BY4741 [EFO:0000098]
- Phenotype : Response To Chemicals
- Condition : ferulic acid (2.5 mM) [PubChem]
- Library : Yeast Inducible CRISPRi Library [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-Mxi repressor domain | Methodology: Inhibition
- Analysis Method : MaGeCK | Number of Hits: 206 | Full Dataset Size: 4,501
- Experimental Setup : Drug Exposure | Duration: 48 Hours
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 AND Score.5 (Log2FC) < -1.0 OR Score.4 (p-Value) < 0.05 AND Score.5 (Log2FC) > 1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative log2fc show increased sensitivity to ferulic acid upon KO
- Genes with a significant positive log2fc show increased resistance to ferulic acid upon KO
Score Distribution
< 0.99994
> 0.000011