CRISPR Screen Dataset
Fatimah J. Al Khazal (2025) - 1-CUSTOM00000028
Title: Unbiased CRISPR Synthetic Lethal Screening for Genetic Vulnerabilities in Succinate Dehydrogenase (SDH)-loss Model of Paraganglioma
Screen Details:
- Screen Rationale : Cell-essential genes in a Sdhb mutant background
- Cell Type : Immortal Mouse Chromaffin Cells
- Cell Line : imCC
- Phenotype : Cell Viability [CMPO:0000013]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 2,171 | Full Dataset Size: 19,648
- Experimental Setup : Timecourse | Duration: 10 Doublings | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (MAGeCK pos score) < 0.05 AND Score.2 (p-Value) < 0.05 OR Score.4 (MAGeCK neg score) < 0.05 AND Score.5 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR screen was carried out in immortalized mouse chromaffin cells to identify potential therapeutic targets for succinate dehydrogenase (SDH)-deficient paraganglioma and pheochromocytoma (PPGL).
Score Distribution
< 0.99998
> 0.000000000008